Cell culture is one of the most expensive and critical aspects of scientific research. Many of our customers often ask us, "Why do you sell cell products when they can grow their own cells and have the capability to pass them on?" It's a valid question. However, it's important to understand that growing and maintaining cells is not as simple as it may seem. Cells grow over time, but they don't just survive—they require strict conditions to thrive. That’s why Hengyuan Xiaobian wants to share some key tips on proper aseptic techniques in cell culture.
First, before starting any experiment, make sure the sterile room and laminar flow hood are properly sterilized using a UV lamp for 30–60 minutes. Then, wipe the workbench with 70% ethanol and turn on the fan for about 10 minutes to ensure clean air circulation. Only one cell line should be handled at a time, even if the medium is the same, to prevent cross-contamination. After the experiment, remove all materials from the workbench and disinfect it again with 70% ethanol. Also, allow at least 10 minutes between operations to maintain sterility.
The work area must remain clean and uncluttered. Place only essential items like pipette racks, tubes, or straws on the bench. Avoid leaving other lab supplies around, as they can disrupt airflow. Always wipe any item with 70% ethanol before bringing it into the sterile environment. Conduct your procedures in the center of the workbench, away from the edges where contamination is more likely.
When handling lab equipment, always be careful. Never touch the tip of a pipette or the mouth of a container. Avoid working directly above an open container to prevent contamination. When opening a bottle, hold the cap with one hand and the body of the bottle with the other, keeping it at a 45-degree angle. Never place the cap face-up on the table, as this increases the risk of microbial entry.
Personal safety is also crucial. Always wear a lab coat and gloves before starting any procedure. If you're working with human-derived cells or viral cultures, use a Class II biosafety cabinet to ensure proper protection. Be cautious of aerosol generation, especially when handling toxic substances like DMSO or TPA. Also, take care to avoid needlestick injuries by handling sharp objects carefully.
Regular maintenance is essential. Check the CO2 cylinder pressure, the CO2 incubator’s temperature, CO2 concentration, and water level in the water tray (which should be filled with sterile water and replaced weekly). Also, monitor the airflow pressure in the laminar flow hood and replace the UV lamp and HEPA filter regularly—pre-filters every 300 hours, and HEPA filters every 3000 hours.
Finally, keep the sink clean by adding a disinfectant like Zephrin (1:750) and changing the water periodically. These small but important steps help maintain a safe and effective cell culture environment. By following these practices, you can significantly reduce the risk of contamination and ensure the success of your experiments.
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