Canine osteopontin (OPN) quantitative detection kit [kit name] canine type I collagen (COL-1) quantitative detection kit (ELISA) [kit use] quantitative detection of canine serum, plasma and related liquid samples I Type collagen (COL-1) content. [Detection principle] The kit adopts double antibody two-step sandwich enzyme-linked immunosorbent assay (ELISA). The standard and the sample to be tested are added to the pre-coated canine type I collagen (COL-1) polyclonal antibody transparent enzyme-labeled plate, and after incubation for a sufficient time, the unbound component is washed and removed, and the enzyme label is added. The working fluid, after incubation for a sufficient period of time, is washed to remove unbound components. Substrate A and B were sequentially added, and the substrate (TMB) was converted to a blue product under the catalysis of horseradish peroxidase (HRP), which turned yellow under the action of acid, and the color depth was compared with canine type I collagen in the sample. The protein (COL-1) concentration was positively correlated, and the OD value was measured at a wavelength of 450 nm. The content of canine type I collagen (COL-1) in the sample was calculated based on the OD value of the standard and the sample.
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